The former Real Madrid and Manchester United star, now at Saudi Arabian side Al-Nassr, is set to appear at a record sixth World Cup.
Portugal and Ronaldo, the all-time leading men’s international goalscorer with 143 goals, have never won the competition.
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Ronaldo could face former Barcelona rival Lionel Messi, who is also set to play at a sixth World Cup after leading Argentina to glory four years ago in Qatar.
The Portuguese forward avoided a three-match ban despite being sent off against the Republic of Ireland in their last qualifying match for an elbow.
Spanish coach Martinez selected a 27-man squad with no major surprises, including Ronaldo’s Al-Nassr team-mate Joao Felix.
Paris Saint-Germain stars Vitinha, Joao Neves, Nuno Mendes and Goncalo Ramos are also included, along with Manchester United playmaker Bruno Fernandes and a contingent from Manchester City, including Bernardo Silva.
In the run-up to the tournament Portugal will face Chile and Nigeria in friendlies, before their World Cup campaign begins against DR Congo on June 17.
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Portugal will also face Uzbekistan and Colombia in Group K on June 23 and 27 respectively.
Portugal 27-man squad list:
Goalkeepers: Diogo Costa (Porto), Jose Sa (Wolves/ENG), Rui Silva (Sporting Lisbon), Ricardo Velho (Genclerbirligi/TUR)
Defenders: Diogo Dalot (Manchester United/ENG), Matheus Nunes, Ruben Dias (Manchester City/ENG), Nelson Semedo (Fenerbahce/TUR), Joao Cancelo (Barcelona/ESP), Nuno Mendes (PSG/FRA), Goncalo Inacio (Sporting Lisbon), Renato Veiga (Villareal/ESP), Tomas Araujo (Benfica)
Midfielders: Ruben Neves (Al-Hilal/KSA), Samu Costa (Mallorca/ESP), Joao Neves, Vitinha (PSG/FRA), Bruno Fernandes (Manchester United/ENG), Bernardo Silva (Manchester City/ENG)
Attackers: Cristiano Ronaldo, Joao Felix (Al Nassr/KSA), Francisco Trincao (Sporting Lisbon), Francisco Conceicao (Juventus /ITA), Pedro Neto (Chelsea/ENG), Rafael Leao (AC Milan/ITA), Goncalo Guedes (Real Sociedad/ESP), Goncalo Ramos (PSG/FRA)
–AFP
Photo: REUTERS/Bernadett Szabo